產品名稱:anti-β-Actin Rabbit Polyclonal antibody(兔多(duo)克隆抗體)
產品型號:K001527P
產品特點:anti-β-Actin Rabbit Polyclonal antibody(兔多克隆抗體(ti)(ti))在(zai)Western Blotting中(zhong)(zhong)使用內(nei)參(can)(can)其實就(jiu)是在(zai)WB過程中(zhong)(zhong)的(de)另外用內(nei)參(can)(can)對(dui)應(ying)的(de)抗體(ti)(ti)檢(jian)測內(nei)參(can)(can),這樣(yang)在(zai)檢(jian)測目的(de)產物的(de)同時可(ke)以(yi)檢(jian)測內(nei)參(can)(can)的(de)表達(da),由于(yu)內(nei)參(can)(can)在(zai)各(ge)組織(zhi)和細胞中(zhong)(zhong)的(de)表達(da)相對(dui)恒定(ding),借(jie)助檢(jian)測每個樣(yang)品內(nei)參(can)(can)的(de)量就(jiu)可(ke)以(yi)用于(yu)校(xiao)正上(shang)樣(yang)誤差,這樣(yang)半定(ding)量的(de)結果(guo)才(cai)更為可(ke)信。
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英文名稱 anti-β-Actin Rabbit Polyclonal antibody
別 名 Actin
應(ying) 用 WB
規 格 100ul
稀釋(shi)比例 WB:1:1000
交叉(cha)反應(ying) human,mouse
GeneID 60
蛋白分子量 42kDa
保(bao) 存 Store at -20°C. Avoid freeze / thaw cycles.Stable for one year from the date of shipment.
儲(chu)存液 PBS, pH 7.4, containing 0.02% sodium azide as Pretive and 50% Glycerol
純化方法 Affinity purification
亞 型 unknown
免疫原 Synthetic Peptide
性 狀 Lyophilized or Liquid
Swiss Prot P60709
克隆類型 Polyclonal Antibody
宿 主 Rabbit
背(bei)景資(zi)料 Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues.
說明(ming)沒有
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Beijing Solarbio Science & Technology Co., Ltd
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Http://www.solarbio。。com
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