產品名稱:G-418 遺傳霉(mei)素硫酸鹽
產品型號:G8161
產品特點:G-418 遺傳霉素硫酸鹽貨號:G8161規格:100mg分子式:C 20 H 40 N 4 O 10 ·2H 2 SO 4分子量:692.71儲存條件:2-8℃外觀(性狀):白色粉末。溶解性:可溶于水、甲醇、緩沖液和培養基。單位:支
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G-418 遺傳霉素硫酸鹽
貨號:G8161
規格:100mg
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《Tumour necrosis factor‐α‐induced protein 8‐like 2 is a novel regulator of proliferation, migration, and invasion in human rectal adenocarcinoma cells》 作者:Dong‐Dong Wu Shi‐Yu Liu Ying‐Ran Gao Dan Lu Ya Hong Ya‐Ge Chen Peng‐Zhen Dong Da‐Yong Wang Tao Li Hui‐Min Li Zhi‐Guang Ren Jian‐Cheng Guo Fei He Xue‐Qun Ren Shi‐Yong Sun Shao‐Feng Duan Xin‐Ying Ji 期刊:Journal of cellular and molecular medicine 影響因子:4.302 PMID:30637920
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說明:
真核表(biao)達篩(shai)(shai)選用抗(kang)生素,可(ke)以用于(yu)篩(shai)(shai)選表(biao)達特定(ding)抗(kang)性基因的穩定(ding)細(xi)胞(bao)(bao)株。篩(shai)(shai)選哺(bu)乳動物細(xi)胞(bao)(bao),推薦(jian)(jian)起(qi)始篩(shai)(shai)選濃(nong)度(du)為(wei)400微(wei)(wei)克/毫升(sheng)(sheng);篩(shai)(shai)選植物細(xi)胞(bao)(bao),推薦(jian)(jian)起(qi)始篩(shai)(shai)選濃(nong)度(du)為(wei)10微(wei)(wei)克/毫升(sheng)(sheng);篩(shai)(shai)選酵母,推薦(jian)(jian)起(qi)始濃(nong)度(du)為(wei)500微(wei)(wei)克/毫升(sheng)(sheng)。根據起(qi)始濃(nong)度(du)的效果可(ke)以再適(shi)當(dang)升(sheng)(sheng)高或降低G-418的使用濃(nong)度(du)。
說明:
真核表達篩選用抗(kang)生素,可(ke)以(yi)用于篩選表達特定抗(kang)性基(ji)因的(de)穩定細胞(bao)株。篩選哺乳動物(wu)細胞(bao),推(tui)薦(jian)起(qi)(qi)始(shi)(shi)篩選濃(nong)(nong)度(du)(du)為(wei)(wei)400微克(ke)(ke)/毫升;篩選植物(wu)細胞(bao),推(tui)薦(jian)起(qi)(qi)始(shi)(shi)篩選濃(nong)(nong)度(du)(du)為(wei)(wei)10微克(ke)(ke)/毫升;篩選酵母,推(tui)薦(jian)起(qi)(qi)始(shi)(shi)濃(nong)(nong)度(du)(du)為(wei)(wei)500微克(ke)(ke)/毫升。根據起(qi)(qi)始(shi)(shi)濃(nong)(nong)度(du)(du)的(de)效果(guo)可(ke)以(yi)再適當升高(gao)或(huo)降(jiang)低G-418的(de)使用濃(nong)(nong)度(du)(du)。
特(te)性:
可溶(rong)于(yu)水、甲(jia)醇(chun)、緩沖(chong)液(ye)和(he)培養(yang)基。溶(rong)解后,0.22μm過濾除(chu)菌,分(fen)成(cheng)小包裝,4℃可保存(cun)(cun)12個月(yue),-20℃保存(cun)(cun)時間更長。常用的儲(chu)存(cun)(cun)液(ye)使(shi)用100mMHEPES(pH7.3)配制,濃度(du)為50mg/ml。使(shi)用HEPES配制的好(hao)處(chu)是: 加入(ru)儲(chu)存(cun)(cun)液(ye)不會改變培養(yang)體系(xi)的pH值。
性狀:
白色(se)粉(fen)末,硫(liu)酸(suan)鹽溶于水、甲醇。
描述:
G418是(shi)一種氨基(ji)(ji)(ji)糖苷類(lei)抗生(sheng)(sheng)素(su) ,這(zhe)種氨基(ji)(ji)(ji)糖類(lei)抗生(sheng)(sheng)素(su)的(de)結構和新(xin)霉(mei)素(su)、慶大霉(mei)素(su)、卡那霉(mei)素(su)相似,在分子(zi)遺傳(chuan)試驗中(zhong),是(shi)穩(wen)定轉(zhuan)染常用的(de)抗性(xing)(xing)篩(shai)選試劑。它通過(guo)抑制轉(zhuan)座子(zi)Tn601,Tn5的(de)基(ji)(ji)(ji)因,干擾核(he)(he)糖體(ti)功能(neng)而阻(zu)斷蛋白質合成,對(dui)原核(he)(he)和真核(he)(he)等(deng)細(xi)胞產(chan)生(sheng)(sheng)毒素(su) ,包(bao)括細(xi)菌、酵母、植(zhi)物和哺乳動(dong)物細(xi)胞 ,也包(bao)括原生(sheng)(sheng)動(dong)物和蠕蟲。當neo基(ji)(ji)(ji)因被整合進真核(he)(he)細(xi)胞DNA后(hou) ,則(ze)能(neng)啟動(dong)neo基(ji)(ji)(ji)因編碼的(de)序列轉(zhuan)錄為mRNA ,從而獲得抗性(xing)(xing)產(chan)物氨基(ji)(ji)(ji)糖苷磷酸轉(zhuan)移酶(mei)的(de)高效表達 ,使(shi)細(xi)胞獲得抗性(xing)(xing)而能(neng)在含有 G418的(de)選擇(ze)性(xing)(xing)培養基(ji)(ji)(ji)中(zhong)生(sheng)(sheng)長
用途:
靠干擾核(he)糖體(ti)功能(neng)阻(zu)斷(duan)多肽合成。不是(shi)常用(yong)的(de)標準抗(kang)生素,常用(yong)于選擇新霉素抗(kang)藥基因(yin)轉染的(de)原核(he)和真核(he)細胞(bao)。在(zai)基因(yin)轉移、基因(yin)敲除、抗(kang)性(xing)篩選以及轉基因(yin)動物等(deng)方面得(de)以廣泛應用(yong)
G-418 遺傳霉素硫酸鹽
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